Fat Grafting

  • Autologous Fat Injection Few post-operative problems
    Not dangerous
    Aesthetic or reconstructive purpose
  • Classical technic used since 1994
    Doctor S.R. Coleman (Dermatologist)
    Mainly used in face lift
    Simple protocol:
    Infiltration and sampling
    Centrifugation: 3min 3000 rpm
  • Areas injected with small and large volume
    Wrinkles / Dark circles / Nasolabial folds / Scars
    Breast augmentation / Buttock augmentation
  • Problem?
    Cell viability: 30% to 50% expected average
    Unpredictable results
  • Solution
    Improvement of tissue survival during all steps of treatment





Size of the cannula

Cell death

Injection: Difficult
Material: Compacted
Resorption: +++

Collection of big lobules increases cell death and formation of oil cysts

Cell viability

Injection: Easy
Material: Fluid
Resorption: +

Collection of small lobules facilitates adipose tissue vascularisation

tissue preparation

Tissue Preparation

Washing & centrifugation

  • Washing (saline) + soft centrifugation
  • Balance between fluids removal and oil creation
  • 1 min at 2000 rpm

+ High speed centrifugation increases pro-inflammatory cytokines release and decreases the collagen expression (not shown)

Oil quantity after centrifugation



  • Injection with a ‘retrograde’ method
  • Injection of small quantities in different layers is preferable
  • Macrofill injection cannulas: 1.7mm x 2.1mm x 130mm and 1.7mm x 2.1mm x 200mm
  • Microfill injection cannulas: 20G (⌀ 0.7mm x 0.9mm x 50mm) and 17G (1.2mm x 1.4mm x 100mm)


  • Short infiltration without anaesthetic, if possible
  • Liposuction with a small cannula (with small holes)
  • Low negative pressure
  • Washing and soft centrifugation
  • Injection of small quantities at different layers


  • Single use, sterile, all-in-one kits
  • Complete and efficient protocol: 70 to 90 % fat retention 6 months post surgery
  • Closed circuit: no risk of infection

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